News: Affymetrix

09.09.2010 | Business Wire: Affymetrix to Webcast Presentations at September Financial Conferences | Business Wire

SANTA CLARA, Calif.-(BUSINESS WIRE)-Affymetrix, Inc. (Nasdaq:AFFX) today announced that company management will provide a corporate overview at four financial conferences in September. Morgan Stanley Healthcare Conference, New York, NY on Mon. Sept. 13th at 1:00pm ET R.W. (…)

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[Source: Business Wire]

01.01.2010 | NCBI PubMed: Impact of Moderate Calorie Restriction on the Reproductive Neuroendocrine Axis of Male Rhesus Macaques.

The impact of moderate calorie restriction on reproductive neuroendocrine function was investigated in young adult male rhesus macaques (Macaca mulatta). The animals were subjected to either 30% calorie restriction (CR; n=5), or were fed a standard control diet (CON; n=5), (…)

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[Source: NCBI PubMed]

01.09.2010 | NCBI PubMed: Effects of early neonatal development and delayed feeding immediately post-hatch on the hepatic lipogenic program in broiler chicks.

The embryo to neonate transition is a critical period of development that has significant impact on broiler production. During this time important genetic programs governing metabolism and growth are established. The goal of this work was to study the effects of early (…)

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[Source: NCBI PubMed]

12.01.2011 | NCBI PubMed: Comparison of methyl-DNA immunoprecipitation (MeDIP) and methyl-CpG binding domain (MBD) protein capture for genome-wide DNA methylation analysis reveal CpG sequence coverage bias.

DNA methylation primarily occurs at CpG dinucleotides in mammals and is a common epigenetic mark that plays a critical role in the regulation of gene expression. Profiling DNA methylation patterns across the genome is vital to understand DNA methylation changes that occur (…)

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[Source: NCBI PubMed]

01.07.2010 | NCBI PubMed: Diagnosis and fine localization of deletion region in Wolf- Hirschhorn syndrome patients.

BACKGROUND: Wolf-Hirschhorn syndrome (WHS) results from the partial deletion of 4p. This study aimed to identify and fine map the chromosome deletion regions of Chinese children with Wolf-Hirschhorn syndrome among the developmental delay/mental retardation (DD/MR) patients. (…)

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[Source: NCBI PubMed]

07.09.2010 | NCBI PubMed: Modulation of transcription factor and metabolic pathway genes in response to water-deficit stress in rice.

Water-deficit stress is detrimental for rice growth, development, and yield. Transcriptome analysis of 1-week-old rice (Oryza sativa L. var. IR64) seedling under water-deficit stress condition using Affymetrix 57 K GeneChip(R) has revealed 1,563 and 1,746 genes to be up- and (…)

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[Source: NCBI PubMed]

01.09.2010 | NCBI PubMed: The molecular basis for sonographic cervical shortening at term: identification of differentially expressed genes and the epithelial-mesenchymal transition as a function of cervical length.

OBJECTIVE: The purpose of this study was to determine whether cervical shortening of a ripe cervix at term is associated with changes in the cervical transcriptome. STUDY DESIGN: Sonographically measured cervical lengths and biopsy specimens were obtained from 19 women at (…)

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[Source: NCBI PubMed]

24.08.2010 | NCBI PubMed: Genome wide association for smoking cessation success in a trial of precessation nicotine replacement.

Abilities to successfully quit smoking display substantial evidence for heritability in classical and molecular genetic studies. Genome wide association (GWA) studies have demonstrated SNPs and haplotypes that distinguish successful quitters from individuals who were not able (…)

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[Source: NCBI PubMed]

07.09.2010 | NCBI PubMed: An ANOCEF genomic and transcriptomic microarray study of the response to radiotherapy or to alkylating first-line chemotherapy in glioblastoma patients.

ABSTRACT: BACKGROUND: The molecular characteristics associated with the response to treatment in glioblastomas (GBMs) remain largely unknown. We performed a retrospective study to assess the genomic characteristics associated with the response of GBMs to either first-line (…)

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[Source: NCBI PubMed]

06.09.2010 | NCBI PubMed: miR-145-dependent targeting of Junctional Adhesion Molecule A and modulation of fascin expression are associated with reduced breast cancer cell motility and invasiveness.

Micro RNAs are small non-coding RNAs, which regulate fundamental cellular and developmental processes at the transcriptional and translational level. In breast cancer, miR-145 expression is downregulated compared with healthy control tissue. As several predicted targets of (…)

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[Source: NCBI PubMed]

09.09.2010 | BioSpace: Affymetrix (Santa Clara, California) (AFFX) to Webcast Presentations at September Financial Conferences

SANTA CLARA, Calif.-(BUSINESS WIRE)-Affymetrix, Inc. (Nasdaq:AFFX) today announced that company management will provide a corporate overview at four financial conferences in September.

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[Source: BioSpace]

01.09.2010 | NCBI PubMed: Genome-wide association study with DNA pooling identifies variants at CNTNAP2 associated with pseudoexfoliation syndrome.

Genetic and nongenetic factors contribute to development of pseudoexfoliation (PEX) syndrome, a complex, age-related, generalized matrix process frequently associated with glaucoma. To identify specific genetic variants underlying its etiology, we performed a genome-wide (…)

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[Source: NCBI PubMed]

05.03.2010 | NCBI PubMed: A Method to Detect Differential Gene expression in Cross-Species Hybridization Experiments at Gene and Probe Level.

MOTIVATION: Whole genome microarrays are increasingly becoming the method of choice to study responses in model organisms to disease, stressors or other stimuli. However, whole genome sequences are available for only some model organisms, and there are still many species whose (…)

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[Source: NCBI PubMed]

27.08.2010 | NCBI PubMed: Identification of a Novel Biomarker SEMA5A for Non-Small Cell Lung Carcinoma in Non-Smoking Women.

BACKGROUND: Although cigarette smoking is the major risk factor for lung cancer, only 7% of female lung cancer patients in Taiwan have a history of smoking. The genetic mechanisms of carcinogenesis in non-smokers are unclear, but semaphorins have been suggested to play a role (…)

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[Source: NCBI PubMed]

01.01.2010 | NCBI PubMed: An ADAM9 mutation in canine cone-rod dystrophy 3 establishes homology with human cone-rod dystrophy 9.

PURPOSE: To identify the causative mutation in a canine cone-rod dystrophy (crd3) that segregates as an adult onset disorder in the Glen of Imaal Terrier breed of dog. METHODS: Glen of Imaal Terriers were ascertained for crd3 phenotype by clinical ophthalmoscopic examination, (…)

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[Source: NCBI PubMed]

01.09.2010 | NCBI PubMed: Cellularity and adipogenic profile of the abdominal subcutaneous adipose tissue from obese adolescents: association with insulin resistance and hepatic steatosis.

OBJECTIVE We explored whether the distribution of adipose cell size, the estimated total number of adipose cells, and the expression of adipogenic genes in subcutaneous adipose tissue are linked to the phenotype of high visceral and low subcutaneous fat depots in obese (…)

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[Source: NCBI PubMed]

27.08.2010 | NCBI PubMed: Massive translational repression of gene expression during mouse erythroid differentiation.

We took advantage of a mouse erythroid differentiation system to determine the relative contribution of transcriptional and translational control during this process. Comparison of expression data obtained with total cytoplasmic mRNAs or polysome-bound mRNAs (actively (…)

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[Source: NCBI PubMed]

27.08.2010 | NCBI PubMed: Integration of transcript expression, copy number and LOH analysis of infiltrating ductal carcinoma of the breast.

ABSTRACT: BACKGROUND: A major challenge in the interpretation of genomic profiling data generated from breast cancer samples is the identification of driver genes as distinct from bystander genes which do not impact tumorigenesis. One way to assess the relative importance of (…)

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[Source: NCBI PubMed]

27.08.2010 | NCBI PubMed: Trophoblast- and Vascular Smooth Muscle Cell-Derived MMP-12 Mediates Elastolysis during Uterine Spiral Artery Remodeling.

During the first trimester of pregnancy, the uterine spiral arteries are remodeled, creating heavily dilated conduits that lack maternal vasomotor control but allow the placenta to meet an increasing requirement for nutrients and oxygen. To effect permanent vasodilatation, the (…)

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[Source: NCBI PubMed]

31.08.2010 | NCBI PubMed: In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells.

An acceleration of differentiation, at the expense of proliferation, is observed after exposure of various biological models to low frequency and low amplitude electric and electromagnetic fields. Following these results showing significant modifications, we try to identify the (…)

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[Source: NCBI PubMed]

27.08.2010 | NCBI PubMed: Molecular diagnosis of known recessive ataxias by homozygosity mapping with SNP arrays.

The diagnosis of rare inherited diseases is becoming more and more complex as an increasing number of clinical conditions appear to be genetically heterogeneous. Multigenic inheritance also applies to the autosomal recessive progressive cerebellar ataxias (ARCAs), for which 14 (…)

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[Source: NCBI PubMed]

27.08.2010 | NCBI PubMed: Simultaneous Mutation Detection in 90 Retinal Disease Genes in Multiple Patients Using a Custom-designed 300-kb Retinal Resequencing Chip.

PURPOSE: To develop a high-throughput, cost-effective diagnostic strategy for the identification of known and new mutations in 90 retinal disease genes. DESIGN: Evidence-based study. PARTICIPANTS: Sixty patients with a variety of retinal disorders, including Leber's congenital (…)

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[Source: NCBI PubMed]

01.09.2010 | NCBI PubMed: Identification of a submicroscopic 3.2 Mb chromosomal 16q12.2-13 deletion in a child with short stature, mild developmental delay, and craniofacial anomalies, by high-density oligonucleotide array-a recognizable syndrome.

Interstitial deletion of 16q has emerged into a recognizable pattern of congenital malformation. We report on a 9-year-old boy with short stature, psychomotor retardation, high forehead, broad flat nasal bridge, hypertelorism, cup-shaped ears, short neck, and a normal (…)

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[Source: NCBI PubMed]

26.08.2010 | NCBI PubMed: Distinct gene expression profiles in subsets of chronic lymphocytic leukemia expressing stereotyped IGHV4-34 B cell receptors.

Background: In chronic lymphocytic leukemia (CLL), multiple subsets of patients exist displaying similar immunoglobulin (IG) gene usage with almost identical complementarity determining region 3 sequences. Among IGHV4-34 cases, two such subsets with "stereotyped" B-cell (…)

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[Source: NCBI PubMed]

01.08.2010 | NCBI PubMed: Analysis of copy number variation in 8,842 Korean individuals reveals 39 genes associated with hepatic biomarkers AST and ALT.

Biochemical tests such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are useful for diagnosing patients with liver disease. In this study, we tested the association between copy number variation and the hepatic biomarkers AST and ALT based on 8,842 (…)

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[Source: NCBI PubMed]

27.08.2010 | Business Wire: Research and Markets: Epigenetics: Tools, Diagnostics, Drugs and World Market | Business Wire

DUBLIN-(BUSINESS WIRE)-Research and Markets (http:/www.researchandmarkets.com/research/910f92/epigenetics_tools) has announced the addition of the "Epigenetics: Tools, Diagnostics, Drugs and World Market" report to their offering. Epigenetics: Tools, Diagnostics, Drugs and (…)

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[Source: Business Wire]

25.08.2010 | NCBI PubMed: Microarray analysis of iris gene expression in mice with mutations influencing pigmentation.

PURPOSE. Several ocular diseases involve the iris, notably including oculocutaneous albinism, pigment dispersion syndrome, and exfoliation syndrome. To screen for candidate genes that may contribute to the pathology of these diseases, genome-wide iris gene expression patterns (…)

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[Source: NCBI PubMed]

25.08.2010 | NCBI PubMed: Pathogenesis of hepatitis E and hepatitis C in chimpanzees: Similarities and differences.

The chimpanzee is the only animal model for investigating the pathogenesis of viral hepatitis types A through E in humans. Studies of the host response, including microarray analyses, have relied on the close relationship between these two primate species: chimpanzee samples (…)

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[Source: NCBI PubMed]

26.08.2010 | Business Wire: Maven Semantic: Tissue Banking Research | Business Wire

DUBLIN-(BUSINESS WIRE)-Maven Semantic (http:/www.mavensemantic.com) announces updates to their Tissue Banking research database. The system uses the context in which a given person appears, using artificial intelligence techniques to find out information not otherwise (…)

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[Source: Business Wire]

24.08.2010 | NCBI PubMed: Molecular analysis of ex-vivo CD133+ GBM cells revealed a common invasive and angiogenic profile but different proliferative signatures among high grade gliomas.

ABSTRACT: BACKGROUND: Gliomas are the most common type of primary brain tumours, and in this group glioblastomas (GBMs) are the higher-grade gliomas with fast progression and unfortunate prognosis. Two major aspects of glioma biology that contributes to its awful prognosis are (…)

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[Source: NCBI PubMed]

24.08.2010 | NCBI PubMed: Oxidant-NO dependent gene regulation in dogs with type I diabetes: impact on cardiac function and metabolism.

ABSTRACT: BACKGROUND: The mechanisms responsible for the cardiovascular mortality in type I diabetes (DM) have not been defined completely. We have shown in conscious dogs with DM that: 1) baseline coronary blood flow (CBF) was significantly decreased, 2) endothelium-dependent (…)

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[Source: NCBI PubMed]

01.09.2010 | NCBI PubMed: Reproducibility and Performance of Virtual Karyotyping With SNP Microarrays for the Detection of Chromosomal Imbalances in Formalin-fixed Paraffin-embedded Tissues.

BACKGROUND: Chromosomal imbalances are commonly seen in cancer and inherited genetic diseases. These imbalances may assist in the diagnosis, prognosis, and/or therapeutic management of certain neoplasms. Several methods for detecting chromosomal imbalances, such as, fluorescent (…)

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[Source: NCBI PubMed]

20.06.2008 | NCBI PubMed: Genome-wide detection of loss of heterozygosity and copy number variation in a human lung large cell carcinoma cell line by affymetrix single-nucleotide polymorphism array 500K.

BACKGROUND: Loss of heterozygosity (LOH) and Copy number copy number variation (CNV) of DNA sequences is a common feature of cancer genomes, which is thought to be linked to tumorigenesis and progression. High-density singlenucleotide polymorphism (SNP) genotyping array are (…)

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[Source: NCBI PubMed]

01.09.2010 | NCBI PubMed: Case-Control Genome-Wide Association Study of Attention-Deficit/Hyperactivity Disorder.

OBJECTIVE: Although twin and family studies have shown attention-deficit/hyperactivity disorder (ADHD) to be highly heritable, genetic variants influencing the trait at a genome-wide significant level have yet to be identified. Thus additional genomewide association studies (…)

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[Source: NCBI PubMed]

24.08.2010 | Business Wire: Research and Markets: Cancer Diagnostic Testing World Markets - With an in Depth Discussion on Over 60 Companies | Business Wire

DUBLIN-(BUSINESS WIRE)-Research and Markets (http:/www.researchandmarkets.com/research/55db96/cancer_diagnostic) has announced the addition of the "Cancer Diagnostic Testing World Markets" report to their offering. Cancer testing is one of the most important growth (…)

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[Source: Business Wire]

20.08.2010 | NCBI PubMed: Acquired genomic copy number aberrations and survival in adult acute myelogenous leukemia.

Genomic aberrations are of predominant importance to the biology and clinical outcome of patients with acute myelogenous leukemia (AML), and conventional karyotype-based risk classifications are routinely used in clinical decision making in AML. One of the known limitations of (…)

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[Source: NCBI PubMed]

23.08.2010 | NCBI PubMed: Transcriptome responses to aluminum stress in roots of aspen (Populus tremula).

ABSTRACT: BACKGROUND: Ionic aluminum (mainly Al3+) is rhizotoxic and can be present in acid soils at concentrations high enough to inhibit root growth. Many forest tree species grow naturally in acid soils and often tolerate high concentrations of Al. Previously, we have shown (…)

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[Source: NCBI PubMed]

18.08.2010 | NCBI PubMed: Effector T cells driving monophasic vs. relapsing/remitting experimental autoimmune uveitis show unique pathway signatures.

Autoimmune diseases often show a relapsing-remitting course. Here we describe characteristics of the autoreactive T cell response in the Lewis rat model of experimental autoimmune uveitis (EAU), a model for the clinical heterogeneity seen in human uveitis. Depending on the (…)

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[Source: NCBI PubMed]

18.08.2010 | NCBI PubMed: Inhibition of Apoptosis by Progesterone in Cardiomyocytes.

Summary While gender-based differences in heart disease have raised the possibility that estrogen (ES) or progesterone (PG) may have cardioprotective effects, recent controversy regarding hormone replacement therapy has questioned the cardiac effects of these steroids. Using (…)

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[Source: NCBI PubMed]

23.08.2010 | NCBI PubMed: Rates of loss of heterozygosity and mitotic recombination in NF2 schwannomas, sporadic vestibular schwannomas and schwannomatosis schwannomas.

Biallelic inactivation of the NF2 gene occurs in the majority of schwannomas. This usually involves a combination of a point mutation or multiexon deletion, in conjunction with either a second point mutation or loss of heterozygosity (LOH). We have performed DNA sequence and (…)

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[Source: NCBI PubMed]

01.01.2010 | NCBI PubMed: A Comparative Study of the Impact of G-Stack Probes on Various Affymetrix GeneChips of Mammalia.

We have previously discovered that probes containing runs of four or more contiguous guanines are not reliable for measuring gene expression in the Human HG_U133A Affymetrix GeneChip data. These probes are not correlated with other members of their probe set, but they are (…)

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[Source: NCBI PubMed]

20.08.2010 | NCBI PubMed: MMP9 polymorphisms and breast cancer risk: a report from the Shanghai Breast Cancer Genetics Study.

In addition to tumor invasion and angiogenesis, matrix metalloproteinase (MMP)9 also contributes to carcinogenesis and tumor growth. Genetic variation that may influence MMP9 expression was evaluated among participants of the Shanghai Breast Cancer Genetics Study (SBCGS) for (…)

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[Source: NCBI PubMed]