This is the RSS news feed of one of our preset news channels on LSIS - The Life Science Infromation Service. Please visit us to find a current view of this one and many more channels via our alerting push services: http://www.lsis.com/channels/?query=129 http://www.lsis.com/goto/r129/rss 2012-05-21T11:11:22+01:00 text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Introduction:? The Hematoflow (Beckman Coulter, USA) is a new automated hematology analyzer, which provides a 16-part white blood cell count (WBC) differential. Methods:? We evaluated the differential WBC count performance of the Hematoflow. 101 blood samples from patients were selected for comparison analysis. Results:? The methodological comparison of the WBC differential parameters of neutrophils, lymphocytes, monocytes and eosinophils showed good correlations among 4 different (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Using 106 samples from patients with an absolute neutrophil count (ANC) less than 2.0 à 10(9)/L, two 5-part differential hematology instruments (Sysmex XE-2100, Sysmex, Kobe, Japan, and Advia 2120i, Siemens Healthcare Diagnostics, Deerfield, IL), two 3-part differential hematology instruments (Sysmex K4500, Sysmex, and Advia 60, Siemens Healthcare Diagnostics), and an automated system for examination of microscopic slides (CellaVision DM96, CellaVision, Lund, Sweden) were compared with a flow (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Medical care cost per capita in developing countries is significantly low, only 1/100 of that of developed countries. In these countries, treatment comes first, without sufficient clinical testing. In emerging countries, on the other hand, economic growth increases medical care cost, enabling more clinical testing; however, in many cases, quality control is not sufficiently performed for economic and technical reasons. To promote evidence-based medical care, it is important to promote (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Hb Acharnes or [?53(D4) Ala - Thr] is a newly discovered unstable hemoglobin variant. It has been reported in very few literatures across the world and no cases have been reported from India till date. Hb Acharnes is known to interact with ?°-thalassemia to produce thalassemia intermedia and heterozygotes may present with borderline HbA2 levels. Here we report a rare case discovered during routine screening of thalassemia and hemoglobinopathies in a 19 year old pregnant lady. To emphasize (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 The study will determine the sensitivity, specificity, negative predictive value and positive predictive value of the OSNA Breast Cancer System by comparing its performance to permanent section Hematoxylin and Eosin (H&E) and IHC staining. |-| Clinical Evaluation of LS-03M/LS-04R for the Detection of Sentinel Lymph Node Metastasis From Breast Cancer |-| Sysmex America, Inc. |-| United States, California John Wayne Cancer Institute Santa Monica, California, United States, 90404 United States, (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 This is a multi-center study designed to evaluate the accuracy and precision of the INRatio Prothrombin Time (PT) Monitoring System, utilizing the INRatio test strip newly designed for low sample volume and heparin insensitivity, when used by trained medical professionals for the quantitative determination of PT and International Normalized Ratio (INR) in fingerstick and venous whole blood from subjects on oral anticoagulation therapy (OAT). These results will be compared to those PT/INR (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 The study will determine the sensitivity, specificity, negative predictive value and positive predictive value of the OSNA Breast Cancer System by comparing its performance to an extensive intraoperative frozen section protocol for Sentinel Lymph Nodes removed during standard Sentinel Lymph Node biopsy procedures from breast cancer subjects. |-| Intraoperative Examination of Sentinel Lymph Nodes: Comparison of the OSNA Breast Cancer System to Extensive Frozen Section Histopathology |-| Sysmex (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 This multi-center study is designed to evaluate the ability of intended lay users (patients on oral anticoagulation therapy, OAT, or their caregivers) to 1) operate the INRatio Prothrombin Time (PT) Monitoring System utilizing the INRatio test strip newly designed for low sample volume and heparin insensitivity, and 2) obtain accurate results for the quantitative determination of International Normalized Ratio (INR) when self-testing using fingerstick capillary blood. Patients will be trained (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 This is a study to evaluate the incidence of sampling bias during pathologic assessment of sentinel lymph nodes (SLN) when they are cut at a certain thickness (1 mm) and the tissue slices are completely analyzed by histopathology (at 200 m sections). The sponsor and investigators would like to determine how often small cancer deposits are present in one slice but not the other. The data will provide an empirical estimate of the incidence of tissue sampling bias inherent in using different (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 This is a multi-center study designed to evaluate the heparin insensitivity of the INRatio Prothrombin Time (PT) Monitoring System, utilizing an INRatio test strip additionally modified for low sample volume. The INRatio test strip is used for the quantitative determination of PT and International Normalized Ratio (INR) results in fingerstick blood from subjects on oral anticoagulation therapy (OAT) with warfarin. This study is designed to evaluate the accuracy of the modified INRatio test (…) text/html http://www.clinicaltrials.gov ClinicalTrials.gov http://www.lsis.com/channels/?query=129 The study will determine the sensitivity, specificity, negative predictive value and positive predictive value of the OSNA Breast Cancer System for patients receiving Sentinel Lymph Node biopsies after receiving neoadjuvant hormonal/chemotherapy as compared to permanent section Hematoxylin and Eosin (H&E) and Immuno-histochemistry (IHC) staining. |-| OSNA Neoadjuvant Feasibility Study in Breast Cancer Patients |-| Sysmex America, Inc. |-| United States, Arizona Arizona Cancer Center Tucson, (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BACKGROUND: Wistar rats are frequently used in toxicologic studies; however, the impact of animal source on hematologic reference intervals (RI) is not known. OBJECTIVE: The purpose of this study was to compare hematologic data of Wistar rats obtained from 2 breeders and to calculate preliminary RI for the Sysmex XT-2000iV hematology analyzer. METHODS: Blood collected in EDTA from 75 10-13-week-old rats (male, n = 38; female, n = 37) from breeder 1 and 60 rats (male, n = 30; female, (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8163490 |-| Title: Method of analyzing methylated DNA |-| Inventors: Sakai; Ayako (Kobe, JP), Kajita; Masahiro (Kobe, JP), Ishihara; Hideki (Miki, JP) |-| Assignee: Sysmex Corporation (Hyogo, JP) |-| The present invention provides a method of analyzing methylated DNA, comprising steps of: (A) treating a DNA-containing sample with a restriction enzyme to obtain a sample containing a DNA fragment; (B) concentrating methylated DNA contained in the sample obtained in step (A) (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Abstract Background: The percentage of hypochromic red cells (% Hypo-He) is now available on the Sysmex analyzer XE 5000 (Sysmex Corporation, Kobe, Japan). In this study the reliability of % Hypo-He in the assessment of iron status in the presence of inflammation is investigated. Methods: Ninety healthy subjects, 91 patients with iron deficiency anemia (IDA) previous to therapy, 65 patients with chronic kidney disease (CKD), 57 patients with anemia of chronic diseases (ACD) and 28 patients (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Abstract Background: In analogy with other areas of laboratory diagnostics, the pre-analytical phase is the leading source of variability also in urinalysis. We carried out a multicentric study for comparing results obtained from first-voided and mid-stream urine samples. Methods: Each of the six hospital-based clinical laboratories participating to this study recruited 50 healthy subjects among laboratory staff and/or their relatives. Two consecutive samples of the first morning micturition (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8160821 |-| Title: Method for supporting a diagnosis of an effect of a treatment by using anthracycline anticancer drugs and a device for supporting a diagnosis for an effect of a treatment by using anthracycline anticancer drugs |-| Inventors: Shibayama; Masaki (Kobe, JP), Ishihara; Hideki (Miki, JP), Matsushima; Tomoko (Kobe, JP), Numada; Shigehiro (Kobe, JP) |-| Assignee: Sysmex Corporation (Hyogo, JP) |-| A method of supporting a diagnosis of an effect of a treatment (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8153418 |-| Title: Apparatus and method for analyzing bacteria |-| Inventors: Kawashima; Yasuyuki (Kobe, JP) |-| Assignee: Sysmex Corporation (Kobe, JP) |-| An apparatus for analyzing bacteria is described that includes an analytic sample preparation section for preparing an analytic sample by treating a specimen so as to generate a morphological difference between Gram-negative bacteria and Gram-positive bacteria, a detector for detecting optical information from each (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Background: Radiation often causes depletion of immunocytes in tissues and blood, which results in immunosuppression. Molecular hydrogen (H2) has been shown in recent studies to have potential as a safe and effective radioprotective agent through scavenging free radicals. This study was designed to test the hypothesis that H2 could protect immunocytes from ionizing radiation (IR). Material/Methods: H2 was dissolved in physiological saline or medium using an apparatus produced by our (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BACKGROUND: The Sysmex XT-2000iV is a hematology analyzer that combines impedance and optical techniques and has been previously validated for dogs. Specific reference intervals (RIs) are useful when interpreting results. OBJECTIVES: The aim of this study was to determine hematologic RIs for the Sysmex XT-2000iV using a large reference population of client-owned clinically healthy blood donor dogs, adopting an indirect sampling method. METHODS: Dogs were screened for breed, size, health, (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 The automated laser-based hematology analyzer Sysmex XT-2000iV? providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats (n = 216), dogs (n = (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 The automated laser-based hematology analyzer Sysmex XT-2000iV? provides a 5-part differential count and specific cytograms that are of great interest for large veterinary laboratories. The aim of the study was to validate the Sysmex XT-2000iV compared to the laser-based hematology analyzer ADVIA 2120 and manual differential in dogs, cats, and horses as well as the impact of anticoagulant (heparin, ethylenediamine tetra-acetic acid [EDTA], and citrate) and storage at 22°C and 4°C. (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Xmn1 polymorphism is a known factor, which increases fetal haemoglobin production. Among the inherited disorders of blood, thalassaemia and Sickle Cell Diseases contributes to a major bulk of genetic diseases in India. Our aim was to verify the role of the Xmn1 polymorphism as a modulating factor in sickle cell patients and frequency of the polymorphism in Indian sickle cell patients. 60 sickle homozygous and 75 sickle beta thalassemia patients were included and 5 ml blood sample was collected (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Adolescent girls are at high risk of developing iron deficiency because of increased iron demands during puberty, menstrual losses, and limited dietary iron intake. This study was carried out to demonstrate the effects of Iron Deficiency Anemia on Audiovisual reaction time in adolescent girls. Adolescent girls between 17-19 years of age with similar socioeconomic background were recruited from college of nursing for the study. They were all screened and categorized into two groups depending on (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Objective: To determine the frequency of adverse effects attributed to Methotrexate (MTX) toxicity and serum minimum toxic concentration with low dose MTX in Rheumatoid Arthritis (RA) patients. Study Design: Cross-sectional observational study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of athology, Rawalpindi, from March 2010 to March 2011. Methodology: One hundred and forty adult patients of RA receiving low dose MTX (10 mg/week) (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Context.-The newest generation hematology analyzer, Sysmex XE-5000 (Sysmex Corporation, Kobe, Japan) is equipped with an improved body fluid analysis mode. Objective.-To evaluate the applicability of the XE-5000 analyzer to white blood cell (WBC) analysis in cerebrospinal fluid (CSF). Design.-A total of 425 routinely collected, consecutive CSF samples were included in the study. For a comparison of total WBC counts, the results of routine chamber counts were grouped into categories of 0 to (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 In cerebrospinal fluid (CSF) analysis, hematology analyzers (HAs) Sysmex() XT-4000i and XE-5000, equipped with flow cytometry (FCM), were used to count cells and differentiate leukocytes into mononuclear and polymorphonuclear cells (MNCs, PMCs) applying body fluid mode. FCM was evaluated with 20 DGKL CSF controls containing viable human leukocytes and erythrocytes. HA values were compared with reference values by Passing/Bablok regression analysis to reveal conformity. Conformity of white (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Introduction:? The aim of this study was to show variability in the measurement of the mean platelet volume (MPV) depending on the instrument used. Methods:? This prospective analysis was carried out to measure MPV with three instruments, in 30 healthy controls and 113 hospital patients. Results:? Firstly, for values in the normal range, the values obtained with the Siemens Advia() 2120 are lower than those given by the Beckman Coulter LH750() (-0.89), which are in turn lower than (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Background: By the end of may 2010, an increase in the number of urines culture-positive for extremely drug-resistant (XDR) Pseudomonas aeruginosa was observed in our 800-bed university hospital. This led to an infection control alert. No epidemiological link between the patients and no increase in the frequency of XDR P. aeruginosa in non-urine samples were observed. Therefore, a pseudo-outbreak due to analytical contamination in the laboratory was rapidly suspected.Methods: A prospective and (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Immunophenotyping of lymphocytes is very essential for evaluation of immune system. Due to the effect of environmental factors and ethnical diversity on immune system, establishment of an internal normal range of lymphocyte subsets is a necessity for each population. The aim of this study was to determine the normal range of T and B lymphocytes, and NK cells in normal Iranian adults. Two hundred and thirty three Iranian normal adult volunteers took part in this study. Complete Blood Count (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 We compared the performance of the basophil count of 3 hematology instruments with a flow cytometric method (FCM) in which CD123 and CD193 were used as basophil markers. By analyzing 112 patient samples, we found the ADVIA 120 (Siemens Healthcare Diagnostics, Deerfield, IL) and CELL-DYN Sapphire (Abbott Diagnostics, Santa Clara, CA) to underestimate the number of basophils by approximately 50% and the Sysmex XE-2100 (Sysmex, Kobe, Japan) and ADVIA to overestimate the basophil count in some (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8082113 |-| Title: Sample analysis system and reagent preparation device |-| Inventors: Nagai; Takaaki (Kobe, JP), Shibata; Masaharu (Kobe, JP), Ueno; Kunio (Kakogawa, JP), Nakanishi; Noriyuki (Kakogawa, JP) |-| Assignee: Sysmex Corporation (Kobe, JP) |-| A sample analysis system includes a reagent preparation unit and a measurement unit. The reagent preparation unit has: a state detector which detects at least one of a state of the reagent preparation unit and a state of (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8078409 |-| Title: External quality control method, external quality control computer, preprocessing device, and nucleic acid detecting device |-| Inventors: Yamasaki; Masatoshi (Sanda, JP), Fujimoto; Keiji (Kobe, JP) |-| Assignee: Sysmex Corporation (Hyogo, JP) |-| An external quality control method, using an external quality control system which comprises an external quality control computer and a plurality of preprocessing devices connected to the external quality (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8076080 |-| Title: Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers |-| Inventors: Tada; Sachiyo (Kobe, JP), Akai; Yasumasa (Kobe, JP), Imura; Yasuyuki (Kobe, JP), Abe; Shigeki (Kobe, JP), Minekawa; Harumi (Otawara, JP) |-| Assignee: Sysmex Corporation (Hyogo, JP) |-| It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 The Sysmex() CS-2100i is a fully automated multiparameter hemostasis analyzer equipped with a photo-optical clot detection unit, a cap-piercing system and a pre-analytical check screens for interfering substances such as bilirubin, lipids and haemolysis (HIL system). It is designed to perform coagulation tests as well as chromogenic and immunologic assays. The aim of the present study was to evaluate its performance. The tests performed were routine coagulation (prothrombin time, activated (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 There are 1.5 million hepatitis B virus (HBV) infected patients in Japan. Anti-viral therapy is important for chronic hepatitis B patients to prevent hepatocellular carcinoma. Recently, HBs antigen (HBsAg) quantification has been reported to be useful for not only HBV screening but also for monitoring of anti-viral treatment. In this paper, we evaluated the clinical utility of quantitative assay of HBsAg by HISCL HBsAg kit. Although there can be a significant difference in age, HBeAg (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Urinary tract infections (UTI) are one of the most common infections in both hospitalised and ambulant patients. Rapid diagnostic of UTIs is necessary to provide early information about the presence of bacteria and the indication to administer an antibiotic therapy. Here we report on a study comparing 3 different rapid automated systems with the semiquantitative plate culture reference method in a university hospital with a highly complex patient population. In total, 2230 urine samples were (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BACKGROUND: Grey eosinophils (GE) reported to occur in Greyhounds, and occasionally in other breeds, have clear granules, probably due to abnormal staining properties. OBJECTIVES: The aims of this study were to investigate the frequency of GE in Greyhounds and 2 other sighthound breeds, and to assess the capacity of the ADVIA 120 and Sysmex XT-2000iV hematology analyzers to correctly identify GE. METHODS: Blood samples from 20 Greyhounds, 29 Italian Greyhounds, and 24 Whippets were analyzed (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BACKGROUND: The need for breed-specific reference intervals (RIs) for Greyhounds has been identified. As Lurchers are a sighthound cross-breed, specific RIs may also be needed for these dogs. Hematologic RIs for Greyhounds and Lurchers using the Sysmex XT-2000iV hematology analyzer have not been established. OBJECTIVES: The aims of this study were to establish RIs for Greyhounds using the Sysmex XT-2000iV, to investigate whether RIs for Greyhound and nonsighthound dogs could be transferred to (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BACKGROUND: The Sysmex pocH-100iV Diff is an impedance hematology analyzer recently introduced for point-of-care use in veterinary practices in Europe. OBJECTIVE: The purpose of this study was to validate the pocH-100iV Diff for analysis of blood samples from dogs, cats, horses, and cattle. METHODS: Fresh EDTA-blood samples from healthy and ill dogs (115), cats (94), horses (91), and cattle (78) were analyzed on the pocH-100iV Diff and the Cell-Dyn 3500. Results of the automated WBC (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BACKGROUND: Most automated hematology analyzers cannot detect canine or feline basophils. However, many veterinary laboratories continue to report basophils as part of the automated 5-part differential leukocyte count for dogs and cats. OBJECTIVES: The study objectives were to evaluate the performance of the Sysmex XT-2000iV, Advia 2120, and CELL-DYN 3500 hematology analyzers in detecting basophils using blood from dogs, cats, and rabbits with basophilia and to investigate the concurrence of (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 False thrombocytopenia may result from platelet aggregation, especially in feline ethylenediamine tetra-acetic acid (EDTA) blood specimens. Citrate, theophylline, adenosine and dipyridamole (CTAD) was added to 46 feline EDTA specimens to test its anti-aggregation action. Platelet aggregation was estimated from blood films and a complete blood count was performed with a Sysmex XT-2000iV analyser. Platelet aggregation score was 2 in 11/46 EDTA tubes and only in one EDTA+CTAD specimen. The (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 The stability of haematological parameters is crucial to guarantee accurate and reliable data for implementing and interpreting the athlete's biological passport (ABP). In this model, the values of haemoglobin, reticulocytes and out-of-doping period (OFF)-score (Hb-60?Ret) are used to monitor the possible variations of those parameters, and also to compare the thresholds developed by the statistical model for the single athlete on the basis of its personal values and the variance of (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8053193 |-| Title: Method and apparatus for judging the presence or absence of cancer cell |-| Inventors: Otomo; Yasuhiro (Kobe, JP), Nakabayashi; Kazuki (Kobe, JP), Shoji; Kayo (Kobe, JP), Takata; Hideki (Kobe, JP) |-| Assignee: Sysmex Corporation (Hyogo, JP) |-| The invention provides a method for judging whether cancer cells are present or not in a sample, comprising a obtaining step of obtaining values related to an expression level of a caner marker gene and a (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 BackgroundFull blood counts are now used as evidence that athletes have used banned blood doping. This has led to legal scrutiny of the efficacy of preanalytical procedures such as specimen homogenisation.AimsTo characterise the impact of different mixing strategies on whole-blood homogeneity.MethodsManual inversion, mechanical mixing and automatic mixing performed by the Sysmex XT-2000i were evaluated.ResultsAutomated mixing by the instrument, or 1 min of mechanical mixing, thoroughly (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 The stability of haematological parameters is crucial to guarantee accurate and reliable data for implementing and interpreting the athlete's biological passport (ABP). In this model, the values of haemoglobin, reticulocytes and out-of-doping period (OFF)-score (Hb-60?Ret) are used to monitor the possible variations of those parameters, and also to compare the thresholds developed by the statistical model for the single athlete on the basis of its personal values and the variance of (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Abstract Background: Nucleated red blood cells (NRBCs) are present in the peripheral blood of several hematological and non-hematological conditions, usually associated with bad prognosis. The lack of an easy, rapid and reliable NRBCs count method did not allow one to know the incidence of NRBCs and to quantify them: the count was usually done during the microscopic revision of a blood smear; this is the reason we found few studies on NRBCs automated count in the literature. The aim of this (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 Abstract Objective. The purpose of the study was to verify the reference intervals for prothrombin time (PT) and activated partial thromboplastin time (APTT), using stored data of ambulatory pre-op subjects with exclusion of certain clinics, according to age and sex. Materials and Methods. Results of test requests (13,600 PT and 14,083 APTT) of subjects aged 15-80 made from outpatient clinics of surgical departments before surgical interventions in 2008 were retrieved from the electronic (…) text/html http://www.uspto.gov/ USPTO http://www.lsis.com/channels/?query=129 United States Patent: 8046043 |-| Title: Extraction device, analyzer, extraction method, and analysis method |-| Inventors: Asano; Kaoru (Kobe, JP), Maekawa; Yasunori (Kobe, JP), Okada; Seiki (Kobe, JP) |-| Assignee: Sysmex Corporation (Kobe, JP) |-| An extraction device for extracting an analyte from living tissue is disclosed that comprises a power source; a first electrode and second electrode connected to the power source; and a holding unit for holding a collecting medium for collecting (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 One-step nucleic acid amplification (OSNA, Sysmex, Kobe, Japan) offers an excellent opportunity for accurate exhaustive sentinel lymph node (SLN) examination in breast cancer patients. Calibrated with conventional postoperative histology, this molecular technique yields comparable results intraoperatively, expressed as micrometastasis, macrometastasis or no metastasis depending on the CK19 mRNA copy number amplified in SLN lysates. We applied OSNA to detect metastasis in 810 SLNs from 367 (…) text/html http://www.ncbi.nlm.nih.gov NCBI PubMed http://www.lsis.com/channels/?query=129 An abstract that appeared on page 1319 of the September 2010 issue (Ciarlini PD, Sandhaus LM, Kidric DM, O'Riordan M. Automated cerebrospinal fluid cell counts using the Sysmex XE-5000: Is it time for new reference ranges? [CAP abstract 30, session 200] Arch Pathol Lab Med. 2010;134[9]:1319) included a financial interest disclosure that Dr. Linda M. Sandhaus received honoraria from Sysmex America, Inc, in support of her work on this abstract. The speaker honoraria Dr. Sandhaus received from (…)